Abstract
Time-Temperature Indicator (TTI) is an intelligent or smart package. It is a simple device that can be attached to food packaging to indirectly reflect the full or partial temperature history and quality status of the food product. TTI is utilized widely throughout food processing and distribution of cold chain. An alkaline lipase TTI has been developed, which based on the reaction between alkaline lipase and glycerol tributyrate. Experiments under several isothermal conditions (1.3°C，6.1°C，10.9°C，15.5°C，20.6°C，25.4°C) were conducted and the Arrhenius parameters of the TTI were derived. The developed TTI chemical reaction system showed an Arrhenius behavior, the activation energy was 45.07±2.25 kJ/mol.
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