DYNAMICS OF THE TISSUE SYSTEM OF PLASMINOGEN REGULATORS IN CUTANEOUS MELANOMA WITH CHRONIC PAIN IN FEMALE MICE

Abstract

Background. Chronic pain is a pathologic process changing the whole body metabolism. Objective: determination of plasminogen regulators in tissues of cutaneous melanoma and in the skin tissues not associated with the tumor in female mice in the dynamics of the growth of experimental melanoma with chronic neurogenic pain. Design and methods . в16/F10 melanoma with chronic pain was reproduced in female С57вL/6 mice. Plasmin-alpha2-antiplasmin complex (PAP), urokinase (pro-uPA and uPA) and tissue (pro-tPA and tPA) plasminogen activators, the uPA (uPAR) receptor and serpin-1 (PAI-1) were determined by ELISA using standard test systems. Results were analyzed using the Statistica 10 program. Results. Chronic neurogenic pain was accompanied by significant changes in the fibrinolysis components in the skin of female С57вL/6 mice. After the в16/ F10 melanoma transplantation (1-3 weeks), skin and tumor tissues showed higher levels of рар and the uPAR receptor (p<0.05), decreased levels of pro-uPA and uPA, pro-tPA and tPA, and PAI-1 (p<0.05), compared to the comparison group (standard melanoma model). Conclusion. Analysis of the state of the tissue fibrinolysis in the skin and tumors of female С57вL/6 mice demonstrated that during the tumor formation with chronic neurogenic pain, plasmin, tPA, uPA, uPAR and PAI-1 play a leading role in the rapid growth and increased malignancy of melanoma. The study of the proposed model will make it possible to clarify many questions of carcinogenesis and the generalization of the malignant process. Studying the model can clarify many issues of the carcinogenesis and progression of the malignant process.