Abstract
CRISPR-Cas effector complexes became widely used in a broad range of genomeengineering applications. Due to simplified cellular delivery, miniature effectorcomplexes such as Cas12f1 with only ∼400-500 aa became of interest and theirgenome editing activity was only recently demonstrated. On the mechanistic level, theprocesses underlying target recognition and DNA cleavage have, however, not beenresolved.Here, we obtain mechanistic insight for Cas12f1 from Syntrophomonas palmitatica.Using mass photometry we characterized the complex composition of the Cas12f1-RNA-complexes.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
