Dynamics of platelets functional activity data in patients after infrarenal aortic aneurysm stentgrafting

Abstract

It is known that hemostatic, immune and autoimmune reactions can play a role in the development and progression of abdominal aortic aneurysms (AAA), including the activation of platelets and CEC However the role of those in pathogenesis of AAA remains unclear.The aim of this study was to study the influence of EVAR on functional activity of platelets and number of circulating endothelial cells (CEC) in comparison with small abdominal aortic aneurysms – AAA (less than 5 cm)Material and methods. Platelets activity has been analyzed due to the assessment of quantity of GP IIb/IIIa receptors and expression of P-selectin. CD14-FITC/CD16-PE/ HLADR-PC5/CD45-PC7 has been used to assess the monocytes activity CEC were counted by flow cytometry in blood samples of patients before EVAR, within 4 weeks and in 18 months after the operation in comparison with non-operated patients due to small diameter AAA. Markers (CD146 + CD45) were used to identify CEC.Results. The amount of Р-selectin, СD62 in patients with small aneurysms (SAAA) in comparison of aneurysms more the 5 cm (BAAA). has been increased (р<0,05). There was the statistically significant difference in amount of GP IIb/IIIa in SAAA and BAAA(р=0,04). The number of monocytes were less in SAAA Positive correlation between CEC and monocytes and between the size of aneurysms in BAAA before the operation have been revealed. We found the positive correlation between CEC number and P-selectin (CD62), in patients at early postoperative period. The significant difference (р=0,02) (GP) IIb/IIIa receptors in patients before and at late postoperative period has been found ><0,05). There was the statistically significant difference in amount of GP IIb/IIIa in SAAA and BAAA(р=0,04). The number of monocytes were less in SAAA Positive correlation between CEC and monocytes and between the size of aneurysms in BAAA before the operation have been revealed. We found the positive correlation between CEC number and P-selectin (CD62), in patients at early postoperative period. The significant difference (р=0,02) (GP) IIb/IIIa receptors in patients before and at late postoperative period has been found.Conclusion. The change in the quantitative characteristics of CEC, platelets and monocytes during the growth of AAA confirms the idea about the activation of innate immunity as an important pathogenetic link in the development of this disease, closely associated with the destruction of the vascular wall and, in particular, of its endothelial lining. The obtained data partly explain the unsatisfactory long-term results of the EVAR and provide an opportunity for developing new approaches for prevention of postoperative complications and the treatment of AAA.