Dynamics of colonization by Aggregatibacter actinomycetemcomitans in aggressive periodontitis families

Aurélio Amorim Reis,Enilson A Sallum,Gabriela Martin Bonilha,Larissa Bizarre,Mabelle Monteiro,Marcio Casati,Renato Correa Viana Casarin

doi:10.20396/revpibic262018513

Abstract

Aggressive periodontitis(AgP) is a disease characterized by the presence of periodontal pathogens and family aggregation of cases, possibly related to the vertical transmission of microorganisms. Thus, the project has evaluated the pattern of colonization of some pathogens in children of parents affected by generalized aggressive periodontitis, compared to children of periodontally healthy parents. Five edentulous babies and 15 primary, mixed and permanent dentate children from families with parents affected by AgP were selected and, by a age and gender-matched approach, similar population from eriodontally heath individuals were also selected. From all subjects, saliva was collected, had a DNA extracted and sequenced for Aggregatibacter actinomycetemcomitans (Aa), Porphyromonas gingivalis (Pg), Treponema denticola (Td), Tannerella forsythia (Tf) identification. Results showed a higher prevalence in AgP-family children regarding Td and Tf. denticola in mixed and permanent dentition (p<0.05). In conclusion,the presence of disease in parentes negatively affectthe oral microbiota of their descendants.

Highlights

Results and Discussion-Aggressive group: Families that with aggressive periodontitis and have at least 01 child aged 0 to 18 years. -Health group: Families who both parents have periodontal health and have a child aged 0 to 18 years.o Saliva samples were collected, DNA extracted from it, 16S-Illumina MISeq Gene Sequencing was ligated.Table 1 shows characteristics such as gender and age of the population of our sample, in the health and aggressive groups, confirming similar distribution (p>0.05).Chart 1 shows the presence of pathogens in each dentition in the different groups
The familial aggregation is an important characteristic of Aggressive periodontitis (AgP) and this can be associated to vertical transmission of microorganisms, genetic factors related to immunoinflammatory response and to susceptibility to disease.[2 3 4]
The general objective of the research project was to evaluate the dynamics of colonization by Aa and other pathogens in the saliva of children and adolescents belonging to families with a history of aggressive periodontitis, comparing the children of healthy periodontal families

Summary

Introduction

Results and Discussion-Aggressive group: Families that (or at least one of the spouses) with aggressive periodontitis and have at least 01 child aged 0 to 18 years. -Health group: Families who both parents have periodontal health and have a child aged 0 to 18 years.o Saliva samples were collected, DNA extracted from it, 16S-Illumina MISeq Gene Sequencing was ligated.Table 1 shows characteristics such as gender and age of the population of our sample, in the health and aggressive groups, confirming similar distribution (p>0.05).Chart 1 shows the presence of pathogens in each dentition in the different groups. Aggressive periodontitis (AgP) is an immunoinflammatory disease of the periodontium that affects young and systemically healthy individuals[1]. The familial aggregation is an important characteristic of AgP and this can be associated to vertical transmission of microorganisms, genetic factors related to immunoinflammatory response and to susceptibility to disease.[2 3 4] The general objective of the research project was to evaluate the dynamics of colonization by Aa and other pathogens in the saliva of children and adolescents (children 0 to adults up to 18 years old) belonging to families with a history of aggressive periodontitis, comparing the children of healthy periodontal families.

Results

Conclusion