Dynamic Effects of Low-level 2-nitro4'-hydroxydiphenylamine on the Induction of HSP70 in the Fish Livers and Brains

Abstract

Carassius auratus was chosen as the experimental materials to study the dynamic effects of 1 mg/L 2-nitro-4' -hydroxydi-phenylamine on the induction of HSP70 in the fish livers and brains. Using the SDS-PAGE and Western Blotting techniques, the expression level of HSP70 in the fish livers and brains was evaluated. As shown from the results, 2-nitro-4' -hydroxydiphenylamine treatment resulted in a time-dependent elevation in HSP70 expression at 2, 4, 7 , 10 and 16 days exposure. Comparing with the control group,the HSP70 in the fish brains and livers began to express significantly after being exposed for 4 days and 7 days respectively. The expression level of HSP70 was enhanced with the exposure days increasing and showed a good time-effect relationship. Results from this study suggest that HSP70 as an environmental biomarker can be a sensitive and rapid indicator in the aquatic ecosystem.