Abstract
Dynamic fluctuations in the conformation of λ-phage DNA have been observed in a fiber optic based light trap. The light force is used to confine a polystyrene bead (diameter, 0.5μm - 2.8μm) in three dimensions at a fixed distance from a flat surface coated with anti-digoxygenin antibody. The DNA is fixed to this bead through a biotin - streptavidin linkage on the 3′end, whereas a digoxygenin label is present on the opposing 5′. Through this simple assay, the dynamics of the free ends have been quantified for various times and separation distances. The dynamics have been observed using a high frame rate camera and the intercalating fluorophore, YOYO-1. In contrast to a conventional optical trap, this trap lacks a highly focused beam, greatly reducing localized heating and the associated risk to the biological specimen which could potentially cause error in this type of measurement.
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