Dynamic changes of laboratory parameters of rats with type 2 diabetes and insulin resistance: defining their role in development of traditional Chinese medicine syndrome

Abstract

To observe the development of Chinese medicine syndromes of rats with type 2 diabetes and insulin resistance by detecting dynamic changes of laboratory parameters. A total of 110 male Sprague-Dawley rats were randomly divided into control group (n=50) and model group (n=60) to observe the correlation between laboratory parameter changes and development of Chinese medicine syndromes. A rat model of type 2 diabetes and insulin resistance was established by feeding rats with high-calorie and high-fat diet together with peritoneal injection of streptozocin (30 mg/kg, once). At the end of 0, 6, 8, 10 and 12 weeks, 10 rats were randomly selected form each group and blood was taken from common carotid artery to detect contents of total cholesterol (TC), triacylglycerol (TAG), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and leptin in serum, cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) contents in plasma and whole blood and plasma viscosities. Compared with the control group, concentrations of TNF-α, IL-6, leptin and cAMP and ratio of cAMP/cGMP increased and content of cGMP decreased markedly from the 6th week to the 12th week. Concentrations of TC, TAG and LDL-C increased from the 8th week to the 12th week, while HDL-C decreased from the 10th week to the 12th week. Whole blood and plasma viscosity values under different shear rates increased significantly from the 10th week to the 12th week. Based on the understanding of markers which have close relationship with Chinese medicine syndromes of dual deficiency of qi and yin, turbid phlegm and blood stasis, combining with the dynamic changes of appearance behavior and laboratory parameters of this animal experiment, the authors speculate the development characteristics of Chinese medicine syndromes in rats with type 2 diabetes. The dynamic progress of Chinese medicine syndromes and pathology varies from dual deficiency of qi and yin to dual deficiency of qi and yin with turbid phlegm, and at last to the combination of dual deficiency of qi and yin, turbid phlegm and blood stasis.