Dynamic change of HMGB1 in acute liver failure rat after transplantation of microencapsulated hepatocytes

Abstract

Objective To detect hepatic function and high mobility group box 1(HMGB1)in dynamic state after microencapsulated hepatocytes transplating into rats with acute liver failure(ALF). Methods We made the model of ALF with D-GalN and assigned the models to 3 groups randomly: group Ⅰ were made with NS; group Ⅱ were made with gymno-hepatocyte; group Ⅲ were made with microencapsulated hepatocyte. The changes of hepatic function and HMGB1mRNA were detected by semiquantitative RT-PCR at the time of 6 h, 12 h, 24 h, 48 h, 72 h, 120 h and 168 h.In addition, reference value came from 6 normal rats. Results ALT, AST and total bilirubin(Tbil)began to increase at 6 h in 3 model groups, and increased significantly at the time of 24 h( P ＜ 0.05). ALT, AST and Tbil in group Ⅲ were decreased obviously, and there was statistical significance with group Ⅰ and group Ⅱ ( P ＜ 0.05 or P ＜ 0.01). In 3model groups, the expression of HMGB1 mRNA increased obviously at 6 h( P ＜ 0.01)and reached to peak at 12 h. Compared with group Ⅰ ,the expression of HMGB1 mRNA in groupⅡ and groupⅢ descended significantly after 24 h. There was statistical significance between group Ⅲ and group Ⅱ at 72 h and 120 h( P ＜ 0.01). Conclusions HMGB1 are intimately associated with ALF. Hepatocellular transplantation can decrease the expression of HMGB1 mRNA in hepatic tissue of rats with ALF, reduce the inflammation of the liver and improve the prognosis of ALF rats. Key words: Liver failure, acute; Microencapsulation; Hepatocyte transplantation; HMGB1