Dynamic bioavailability of copper in soil estimated by uptake and elimination kinetics in the springtail Folsomia candida

Abstract

This study aimed to assess the bioavailability of copper in soil, by measuring its uptake kinetics into a representative soil invertebrate, the collembolan Folsomia candida. The animals were exposed to 25 or 100 μg Cu g(-1) dry LUFA 2.2 soil at nominal pH(CaCl2) 4.5, 5.5, or 6.5 during 14 days after which they were transferred to clean soil for 14 days elimination. Uptake and elimination rate constants were calculated based on total and extractable soil concentrations and porewater concentrations using one-compartment first-order kinetics modelling. Copper was present in the animals at a basal physiological level of 40-90 μg g(-1)dry weight, on top of this uptake and elimination kinetics were observed. Uptake rates constants varied between 0.02 and 0.17 g(soil) g(animal)(-1) day(-1), being higher at lower exposure level, but did not differ significantly between different soil pH levels. Elimination rate constants ranged between 0.04 and 0.20 day(-1) and were negligible (k(2) < 0.001 day(-1)) at pH 4.5 and 6.5. Multiple linear regressions showed that the pH effect on copper uptake was only significant when taking into account cation exchange capacity, or calcium and dissolved organic carbon levels in the pore water. Copper concentrations in the animals however, never were higher than 185 μg g(-1) dry weight, independent of exposure level and pH, suggesting homeostatic regulation. These results show that the chemical composition of the pore water does affect bioavailability of copper in soil, but that copper uptake in collembolans is dominated by homeostatic regulation rather than by soil properties like pH.