Abstract
To examine the cervical lesions by using the tumor-targeted near-infrared (NIR) fluorescent dyes TMTP1-PEG4-ICG, a dual modal colposcope with visible reflectance imaging and fluorescence imaging was developed. NIR fluorescence imaging and visible light reflectance imaging were integrated together on a colposcope by designing the specific optics and adopting a dual sensor charge coupled device (CCD) camera. Patients with squamous cell carcinoma, adenocarcinoma, cervical intraepithelial neoplasia (CIN) and cervicitis were examined using this dual modal colposcope to validate its potential of cervical cancer detection. Fluorescent dye TMTP1-PEG4-ICG was applied to the cervix 30 minutes before inspection. The fluorescence images were collected after wiping the unbound fluorescent dye using normal saline. Signal to back ratios (SBR) of the fluorescence images were analyzed and compared with the histological analysis. The results suggest that the fluorescent colposcope combined with tumor-specific near-infrared fluorescent dyes TMTP1-PEG4-ICG could help to evaluate cervical lesions in real time.
Highlights
Cervical cancer is a common gynecological malignancy and ranks the fourth for both incidence and mortality among all cancers in female worldwide
Intraoperative NIR fluorescence imaging is widely applied in clinical practices such as sentinel lymph node mapping, tumor imaging and angiography [15]
It was shown that this fluorescent colposcope combined with a tumor-targeting NIR fluorescent dye TMTP1-PEG4-ICG had the potential to display positive fluorescent signal at the lesions for squamous cell carcinoma, adenocarcinoma and cervical intraepithelial neoplasia (CIN)
Summary
Cervical cancer is a common gynecological malignancy and ranks the fourth for both incidence and mortality among all cancers in female worldwide. Improving the rate of early diagnosis of cervical cancer is crucial for the reduction of mortality. Colposcopy, along with colposcopically directed biopsies, is indicated for evaluating women with abnormal screening results. There is a wide variability in the reported sensitivity (ranging 55%-100%) and specificity (ranging 65%-98%) of acetate-indicated visual inspection method [5]. Because this examination relies on the subjective judgement of inspector, the accuracy of the results is highly related to the colposcopists’ experience [6]
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