Abstract
1. 1. The fractionation of the liver of goldfish ( Carassius auratus) was studied, and the properties of the microsomal fraction were examined. 2. 2. The microsomal fraction contained cytochrome P-450 and catalyzed the oxidation of aminopyrine, aniline, 7-ethoxycoumarin and benzo(a)pyrene. 3. 3. The oxidation activities were significantly lower than those of rat liver microsomes. 4. 4. The titration of cytochrome P-450 by potassium cyanide indicated the presence of multiple forms of cytochrome P-450 in goldfish liver microsomes. 5. 5. Feeding of goldfish with 3-methylcholanthrene-containing food greatly induced benzo(a)pyrene hydroxylation activity of the liver microsomes. The Soret peak of the carbon monoxide compound of cytochrome P-450 was shifted from 450 to 448 nm.
Published Version
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