Abstract
We report the draft whole-genome sequences of two Borrelia miyamotoi strains isolated in The Netherlands. Using next-generation sequencing, we determined the complete sequence of the chromosomes and several plasmids. The two strains show a genotype typical of European strains, distinct from the genomes of strains from Asia or the United States.
Highlights
Molecular analysis of the emerging human hard tick-borne pathogen Borrelia miyamotoi (1) is cumbersome, since at present a limited set of isolates are available and have been sequenced (2, 3)
Through an InterProScan v5.23-62.0 search as described by Koetsveld et al (8), we identified 39 coding DNA sequences (CDSs) and 8 pseudogenes of vlp genes and 16 CDSs of variable small protein genes in the NL-IR-1 genome, whereas in the NL-IR-2 genome, we found 37 CDSs and 11 pseudogenes of vlp genes and 14 CDSs of vsp genes
Phylogenetic analysis based on the core gene alignment of the complete chromosome sequences of B. miyamotoi genomes of different origin in GenBank resulted in a separate phylogenetic lineage for the European B. miyamotoi strains (Fig. 1)
Summary
Molecular analysis of the emerging human hard tick-borne pathogen Borrelia miyamotoi (1) is cumbersome, since at present a limited set of isolates are available and have been sequenced (2, 3). Comparison analysis with the previously sequenced genome of CT13-2396 using Mummer v3, with the maxmatch option and minimal clusters of 100 nucleotides, resulted in contigs of both strains including the complete linear chromosomes of 903,844 bp (NL-IR-1) and 905,334 bp (NL-IR-2) and complete plasmids (lp[72], lp6), in combination with the partially sequenced (left part) virulence plasmid lp[41], containing the variable large protein (vlp) delta subfamily after the expression site (7).
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