Abstract
Brazilian purpuric fever is a febrile hemorrhagic pediatric disease caused by Haemophilus influenzae biogroup aegyptius, a bacterium which was formerly associated with only self-limited purulent conjunctivitis. Here, we present draft genomes of strains from five Brazilian purpuric fever cases and one conjunctivitis case.
Highlights
For over a century, Haemophilus influenzae biogroup aegyptius was associated only with seasonal epidemics of self-limited purulent conjunctivitis (“pink eye”)
The DNA libraries were prepared with the Nextera XT DNA library preparation kit (Illumina, CA, USA) and sequenced using the Illumina HiSeq 2000 platform (100-bp paired-end reads)
The Brazilian purpuric fever (BPF)-associated strains were found to be of sequence type 65 (ST65), while the conjunctivitis strain has the ST72 profile
Summary
Haemophilus influenzae biogroup aegyptius was associated only with seasonal epidemics of self-limited purulent conjunctivitis (“pink eye”). Strains stored at – 80°C were grown on chocolate agar at 37°C with 5% CO2. Genomic DNA was extracted using a cetyltrimethylammonium bromide (CTAB) extraction method [5]. The DNA libraries were prepared with the Nextera XT DNA library preparation kit (Illumina, CA, USA) and sequenced using the Illumina HiSeq 2000 platform (100-bp paired-end reads). The number of sequenced reads ranged from 30,479,940 to 53,773,000, representing an extremely high sequencing coverage of 1,604ϫ and 2,830ϫ, respectively (see Table 1 for total reads and genome coverage per sample).
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