Abstract
Suspected Brazilian Purpuric Fever, Brazilian Amazon Region
Highlights
To determine whether recent reports of Brazilian purpuric fever (BPF) were accurate, we reviewed medical records of the hospital in Anajás
All specimens were submitted for bacterial culture in half agar chocolate without bacitracin; serum and cerebrospinal fluid (CSF) were subjected to real-time PCR for detection of Neisseria meningitides, Streptococcus pneumoniae, and Haemophilus influenzae serotypes a, b, c, and d and to conventional PCR for the ompP2 gene of H. influenzae
Because of the remoteness of the outbreak site, samples for bacterial culture were collected on locally available blood agar enriched with rabbit serum without antimicrobial drug–selective agents, rather than on the recommended chocolate agar enriched with horse serum and bacitracin (1)
Summary
To determine whether recent reports of BPF were accurate, we reviewed medical records of the hospital in Anajás. From 2 children with nonfatal illness, we collected blood, serum, conjunctival swabs, and cerebrospinal fluid (CSF). Serum and CSF samples were thawed and refrozen repeatedly for removal of aliquots before testing. Microbiologic and virologic testing was conducted at the Pará State Health Laboratory and Evandro Chagas Institute.
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