Abstract
Uropathogenic Escherichia coli (UPEC) strains are responsible for most cases of urinary tract infections worldwide. We present the draft whole-genome sequence of the UPEC 252 strain, which carries the eae gene that encodes the intimin adhesin. Intimin promotes intimate adherence of enteropathogenic E. coli and enterohemorrhagic E. coli to intestinal cells.
Highlights
Uropathogenic Escherichia coli (UPEC) strains are responsible for most cases of urinary tract infections worldwide
UPEC 252 was stored in lysogeny broth (LB) plus 15% glycerol and kept at – 80°C
The annotation showed that UPEC 252 has 1 transfer-messenger RNA gene, 83 tRNA genes, 8 rRNA genes, and 4,520 coding sequences (CDS)
Summary
Uropathogenic Escherichia coli (UPEC) strains are responsible for most cases of urinary tract infections worldwide. UPEC 252 was stored in lysogeny broth (LB) plus 15% glycerol and kept at – 80°C. One isolated colony obtained on MacConkey agar was cultured overnight in LB at 37°C, and DNA extraction was performed using the Wizard genomic DNA purification kit (Promega USA), following the manufacturer’s recommendations.
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