Abstract
Members of the Marseilleviridae family are large DNA viruses with icosahedral particles that infect Acanthamoeba cells. This report presents a new Marseilleviridae family member discovered in a water/soil sample from a river in Tokyo, named Tokyovirus, with genome size of 370 to 380 kb.
Highlights
Members of the Marseilleviridae family are large DNA viruses with icosahedral particles that infect Acanthamoeba cells
After the supernatant was removed, the pellet was resuspended in 4 ml of peptone yeast extract-glucose (PYG) broth and filtered again (Millex-AA; Millipore)
4 ml of fresh PYG and a 1-ml suspension of amoeba cells were added to this viral solution, which was divided and cultured on 56 wells in a 96-well culture plate at 26°C
Summary
Members of the Marseilleviridae family are large DNA viruses with icosahedral particles that infect Acanthamoeba cells. The Marseilleviridae family is a group of giant viruses with smaller particles of 200-nm diameter and with a genome size of 300 to 400 kb [1,2,3,4,5,6,7,8,9]. This article reports the draft genome of a new virus belonging to the family Marseilleviridae, isolated from the coast of the Arakawa River, located in Tokyo, Japan. It was named Tokyovirus according to the rules of nomenclature for Marseilleviridae.
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