Abstract
Bacillus licheniformis SMIA-2, a thermophilic and thermostable enzyme-producing bacterium, is found to be active against several strains of Staphylococcus aureus and several Bacillus species. Here, we report the 4.30-Mbp draft genome and bioinformatic predictions supporting gene inventories for amylase, protease, cellulase, xylanase, and antimicrobial compound biosynthesis.
Highlights
Genomic DNA was purified from a 12-h culture grown at 50°C in brain heart infusion broth by using the DNeasy blood and tissue kit (Qiagen) following the manufacturer’s protocol for Gram-positive bacterial DNA extraction
Default parameters were used for all software unless otherwise specified
Projects/fastqc) was used to inspect the quality of the sequences, and quality trimming was based on Phred quality scores of 20 with SolexaQA v3.0 [10]
Summary
Draft Genome Sequence of the Thermophilic Bacterium Bacillus licheniformis SMIA-2, an Antimicrobial- and Thermostable Enzyme-Producing Isolate from Brazilian Soil. Samara Pinto Custodio Bernardo,a Albert Remus R. Rosana,b Adriane Nunes de Souza,a Sorina Chiorean,b Meire Lelis Leal Martins,a John C. Vederasb aFood Technology Laboratory, State University of North Fluminense, Campos dos Goytacazes, Rio de Janeiro, Brazil bDepartment of Chemistry, University of Alberta, Edmonton, Alberta, Canada
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