Determination of Total Xylanase Activities of Various Thermophilic Bacteria

Abstract

Enzymes, which have important metabolic functions in living organisms, make important contributions to human beings by using them for different purposes in many areas such as economy, food, agriculture, and industry as part of the daily lives. Today, enzymes, whose production and usage purposes, are increasing, were generally obtained from plant, animal, and microorganisms. In this study; the enzyme producing isolates and their total xylanase activities of various thermophilic bacteria (Bacillus coagulans, Bacillus licheniformis, Bacillus subtilis, Bacillus sp. and Geobacillus kaustophilus) isolated from previously hot springs in Eastern and Southeastern Anatolia regions were determined. In the enzyme activity of the isolates, 47 isolates showed xylanase activity less than 0.1 U/ml, 31 isolates were between 0.1 U/ml and 0.2 U/ml. However, 5 isolates of B. subtilis [BTX3 (0.206 U/ml), BTX6 (0.286 U/ml), BTX22 (0.2 U/ml), BTX27 (0.203 U/ml) and BTX32 (0.206 U/ml)] were exhibited highest activity for xylanase enzyme production.