Abstract
Staphylococcus epidermidis is a native member of the human microbiota. Here, we report the draft genome sequence of S. epidermidis UMB8493, an isolate from a catheterized urine sample from a female with overactive bladder symptoms. The 2.54-Mbp draft genome encodes genes associated with beta-lactam and fosfomycin resistance.
Highlights
Received April 2020 Accepted May 2020 Published 4 June 2020 mra.asm.org 1
The S. epidermidis UMB8493 draft genome sequence is 2,535,022 bp long assembled into 61 contigs with a GC content of 32%, genome coverage of 267ϫ, and N50 score of 88,403 bp
PATRIC detected several genes associated with antibiotic resistance, including blaZ and fosB
Summary
Received April 2020 Accepted May 2020 Published 4 June 2020 mra.asm.org 1 The DNA library was sequenced using an Illumina NextSeq 550 platform, producing 2,584,013 pairs of 150-bp reads. Raw reads were trimmed using Sickle v1.33 (https://github.com/najoshi/sickle) and assembled using SPAdes v3.13.0 with the “only-assembler” option for K values of 55, 77, 99, and 127 (7).
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