Abstract
The draft genome sequence of Saccharomonospora piscinae KCTC 19743T, with a size of 4,897,614 bp, was assembled into 11 scaffolds containing 4,561 open reading frames and a G+C content of 71.0 mol%. Polyketide synthase and nonribosomal peptide synthetase gene clusters, which are responsible for the biosynthesis of several biomolecules, were identified and located in different regions in the genome.
Highlights
The draft genome sequence of Saccharomonospora piscinae Korean Collection for Type Cultures (KCTC) 19743T, with a size of 4,897,614 bp, was assembled into 11 scaffolds containing 4,561 open reading frames and a GϩC content of 71.0 mol%
The type strain of Saccharomonospora piscinae was obtained from the Korean Collection for Type Cultures (KCTC) and grown for 7 days at 37°C on HM medium (4) with 10% salts, under aerobic conditions
Cells were lysed with a mixture of lysozyme and sodium lauryl sulfate, and nucleic acids were extracted with chloroform-isoamyl alcohol (24:1 [vol/vol]), followed by DNA precipitation with ethyl alcohol
Summary
The draft genome sequence of Saccharomonospora piscinae KCTC 19743T, with a size of 4,897,614 bp, was assembled into 11 scaffolds containing 4,561 open reading frames and a GϩC content of 71.0 mol%. The type strain of Saccharomonospora piscinae was obtained from the Korean Collection for Type Cultures (KCTC) and grown for 7 days at 37°C on HM medium (4) with 10% salts, under aerobic conditions. Genomic DNA was isolated as described elsewhere (5). The draft genome sequence of Saccharomonospora piscinae KCTC 19743T was obtained by following a complete-genome shotgun strategy (6) on an Illumina NovaSeq 6000 platform (2 ϫ 150-bp paired-end reads) (Stab Vida, Portugal), with an output of 23,534,814 reads and a sequencing depth of 733ϫ.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
