Draft Genome Sequence of Micromonospora sp. Strain MW-13, a Bacterial Strain with Antibacterial Properties and Plant Growth Promotion Potential Isolated from the Rhizosphere of Wheat in Iran.

Ghazaleh Jahanshah,Tobias Busche,Jörn Kalinowski,Christian Rückert,Henrike Miess,Harald Gross,Hassan Rokni-Zadeh,Steven R Gill

doi:10.1128/mra.01375-18

Abstract

Strain MW13 exhibited broad-spectrum antibacterial activity toward Gram-positive and Gram-negative pathogens. The 7.1-Mb draft genome gives insight into the complete secondary metabolite production capacity and reveals genes putatively responsible for its antibacterial activity, as well as genes which contribute to plant growth promotion.

Highlights

As part of our ongoing efforts to investigate natural products from rhizosphere bacteria, which are relevant in an agricultural or pharmaceutical context [1,2,3,4,5,6], we recently isolated and characterized 80 rhizospheric soil samples from wheat, barley, and clover fields, as well as turf grass and buckthorn trees in the Isfahan province in central Iran
Strain MW13 was grown in 15 ml Trypticase soy broth (TSB) overnight at 30°C on a rotary shaker (180 rpm)
For genomic DNA isolation, the Qiagen genomic DNA purification kit was used in combination with 100/G Genomic-tips, according to the manufacturer’s protocol, except that for the bacterial lysis, the handled volumes were doubled, and the incubation time at 50°C was prolonged until a clear lysate was obtained

Summary

Introduction

As part of our ongoing efforts to investigate natural products from rhizosphere bacteria, which are relevant in an agricultural or pharmaceutical context [1,2,3,4,5,6], we recently isolated and characterized 80 rhizospheric soil samples from wheat, barley, and clover fields, as well as turf grass and buckthorn trees in the Isfahan province in central Iran. Strain MW13 was grown in 15 ml Trypticase soy broth (TSB) overnight at 30°C on a rotary shaker (180 rpm). For genomic DNA (gDNA) isolation, the Qiagen genomic DNA purification kit was used in combination with 100/G Genomic-tips, according to the manufacturer’s protocol, except that for the bacterial lysis, the handled volumes were doubled, and the incubation time at 50°C was prolonged until a clear lysate was obtained.

Objectives

Results

Conclusion