Abstract
Here, we report the draft genome sequence of Microbacterium sp. strain Gd 4-13, isolated from late Pleistocene permafrost of marine origin located on the Gydanskiy Peninsula. Genome sequence analysis was performed to understand strain survivability mechanisms under permafrost conditions and to expand biotechnology applications.
Highlights
Permafrost is the natural repository of a vast pool of ancient viable microorganisms that possess new enzymes for biotechnological applications (1, 2)
Genome annotation revealed a total of 3,268 genes, including 3,216 proteincoding sequences (CDSs), 54 pseudogenes, 1 rRNA operon, 46 tRNAs, 3 noncoding RNAs, and 25 genes encoding endonucleases
Analysis of the annotated proteins against the InterPro protein signature database using InterProScan v.5.23-62.0 revealed that the genome of Microbacterium sp
Summary
Permafrost is the natural repository of a vast pool of ancient viable microorganisms that possess new enzymes for biotechnological applications (1, 2). The draft genome assembly contains 20 contigs with an average coverage of 200ϫ, a total length of 3,429,146 bp, an N50 value of 382,878 bp, and a high GC content of 69.4%. Genome annotation revealed a total of 3,268 genes, including 3,216 proteincoding sequences (CDSs), 54 pseudogenes, 1 rRNA (rrn) operon, 46 tRNAs, 3 noncoding RNAs (ncRNAs), and 25 genes encoding endonucleases.
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