Abstract
The draft genome sequence of Mesorhizobium sp. strain SARCC-RB16n reveals the presence of major symbiotic (nod and nif) and additional plant growth-promoting (PGPR) genes associated with enhanced growth of Aspalathus linearis (Burm. f.) in South Africa. The genome sequence provides vital information for the development of a commercial inoculant for rooibos cultivation.
Highlights
South African legume nodulated by members commonly of both the alpha and beta subgroups of Proteobacteria [1]
The symbiotic properties of Mesorhizobium sp. strain SARCC-RB16n were further investigated by sequencing its genome to determine the underlying genetics of the nodulation and plant growth-promoting (PGPR) traits observed in the greenhouse and field trials
For DNA extraction, a single pure colony was transferred from solid YM agar into sterile yeast mannitol (YM) broth and grown for 48 hours on a rotary shaker at 28°C
Summary
South African legume nodulated by members commonly of both the alpha and beta subgroups of Proteobacteria [1]. Glasshouse nodulation screening, together with several other strains, confirmed strain SARCC-RB16n to be the most competitive nodulator and the most effective nitrogenfixing strain in rooibos [1]. Strain SARCC-RB16n were further investigated by sequencing its genome to determine the underlying genetics of the nodulation and plant growth-promoting (PGPR) traits observed in the greenhouse and field trials.
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