Draft Genome Sequence of Marine-Derived Bacillus subtilis TP-B0611, a Producer of Bacilosarcins and Amicoumacins.

Hisayuki Komaki,Akira Hosoyama,Natsuko Ichikawa,Yasuhiro Igarashi

doi:10.1128/genomea.01134-16

Abstract

Here, we report the draft genome sequence of Bacillus subtilis TP-B0611, which produces the isocoumarin-type compounds bacilosarcin and amicoumacin. The genome encodes three nonribosomal peptide synthetase (NRPS) gene clusters and one hybrid polyketide synthase (PKS)/NRPS gene cluster. The hybrid PKS/NRPS gene cluster was identified to be responsible for the biosynthesis of bacilosarcins and amicoumacins.

Highlights

We report the draft genome sequence of Bacillus subtilis TP-B0611, which produces the isocoumarin-type compounds bacilosarcin and amicoumacin
During the course of screening marine-derived bacteria for new bioactive compounds, Bacillus subtilis TP-B0611 was isolated from intestine content of a sardine (Sardinops melanostictus) collected in Toyama Bay, Japan, and found to produce two new isocoumarin-type compounds, designated bacilosarcins A and B, with herbicidal activity, together with three known isocoumarins, amicoumacins A, B, and C (1)
Bacilosarcins are distinct from amicoumacins due to the presence of 2-hydroxymorpholine moiety, chemical backbones of bacilosarcins and amicoumacins are quite similar

Summary

Introduction

We report the draft genome sequence of Bacillus subtilis TP-B0611, which produces the isocoumarin-type compounds bacilosarcin and amicoumacin. During the course of screening marine-derived bacteria for new bioactive compounds, Bacillus subtilis TP-B0611 was isolated from intestine content of a sardine (Sardinops melanostictus) collected in Toyama Bay, Japan, and found to produce two new isocoumarin-type compounds, designated bacilosarcins A and B, with herbicidal activity, together with three known isocoumarins, amicoumacins A, B, and C (1). Bacilosarcins are distinct from amicoumacins due to the presence of 2-hydroxymorpholine moiety, chemical backbones of bacilosarcins and amicoumacins are quite similar.

Results

Conclusion