Abstract

Campylobacter fetus is a Gram-negative, zoonotic pathogen and a member of the class Epsilonproteobacteria We report the draft genome sequence of C. fetus subsp. fetus CITCf01, isolated from a patient with subacute bacterial endocarditis. CITCf01 grew under aerobic, microaerobic, and anaerobic conditions, and at 42°C, an unusual combination of growth conditions.

Highlights

  • Campylobacter fetus is a Gram-negative, zoonotic pathogen and a member of the class Epsilonproteobacteria

  • CITCf01 grew in a 5% CO2-enriched aerobic environment, microaerobically and anaerobically, at 35°C on Columbia blood agar (CBA) (Oxoid) and on Mueller-Hinton agar

  • Whole-genome sequencing (WGS), and subsequent assembly were performed by MicrobesNG (University of Birmingham, UK) using the following methods

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Summary

Introduction

Campylobacter fetus is a Gram-negative, zoonotic pathogen and a member of the class Epsilonproteobacteria. Subcultures of the positive culture bottles grew microaerobically (at 35°C, 37°C, and 42°C) on selective charcoal-cefoperazone deoxycholate agar (CCDA) (Oxoid). CITCf01 grew in a 5% CO2-enriched aerobic environment, microaerobically and anaerobically, at 35°C on Columbia blood agar (CBA) (Oxoid) and on Mueller-Hinton agar. CITCf01 was cultured microaerobically from frozen stocks on blood-free Campylobacter selectivity agar (Sigma-Aldrich, USA).

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