Abstract
ABSTRACTThe fungus Aspergillus persii strain NIBRFGC000004109 is capable of producing penicillic acid and showed antibacterial activity against various plant-pathogenic bacteria, including Xanthomonas arboricola pv. pruni. Here, we report the first draft whole-genome sequence of A. persii. The assembly comprises 38,414,373 bp, with 12 scaffolds.
Highlights
The fungus Aspergillus persii strain NIBRFGC000004109 is capable of producing penicillic acid and showed antibacterial activity against various plantpathogenic bacteria, including Xanthomonas arboricola pv. pruni
Reads containing the nuclear genome were assembled into 12 scaffolds (501 contigs), with an N50 value of 4.60 Mb, using a mixed pipeline of FALCON, an assembler of PacBio reads, and SOAPdenovo version 2, an assembler of Illumina reads [2]
A total of 12,328 protein-coding genes were identified after gene prediction and annotation based on the AUGUSTUS software [4]
Summary
The fungus Aspergillus persii strain NIBRFGC000004109 is capable of producing penicillic acid and showed antibacterial activity against various plantpathogenic bacteria, including Xanthomonas arboricola pv. pruni. Genomic DNA was extracted from germlings grown in yeast extract broth by shaking at 150 rpm overnight at 23°C, using the DNeasy minikit (Qiagen, Valencia, CA, USA). A total of 24.62 Gb of genome sequences were produced using 1 paired-end and 2 mate pair libraries on HiSeq 2000 (Illumina) and 8 cells on PacBio RSII platforms at the Theragen Etex Bio Institute (Suwon, South Korea), representing 616.57-fold coverage.
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