Abstract
The gastrointestinal tract of the white-throated woodrat Neotoma albigula harbors a diverse microbial population that functions in the degradation of ingested plant secondary compounds. Here, we present the draft genome sequence and annotation of Clostridium sporogenes strain 8-O, a novel oxalate-degrading bacterium isolated from the feces of N. albigula.
Highlights
The gastrointestinal tract of the white-throated woodrat Neotoma albigula harbors a diverse microbial population that functions in the degradation of ingested plant secondary compounds
Dilutions were plated onto brain heart infusion (BHI) agar supplemented with 20 mM sodium oxalate and 1 g/L calcium chloride
To evaluate in-vitro oxalate degradation, isolates were inoculated into BHI broth supplemented with 20 mM sodium oxalate and incubated for 48 h
Summary
The gastrointestinal tract of the white-throated woodrat Neotoma albigula harbors a diverse microbial population that functions in the degradation of ingested plant secondary compounds. Neotoma albigula consumes a diet consisting primarily of Opuntia cactus which is rich in oxalate (1). N. albigula is not capable of metabolizing oxalate, and relies on bacterial populations in their gut to degrade oxalate. Previous work has demonstrated that N. albigula houses multiple oxalate-degrading taxa throughout its gastrointestinal tract, and many of these oxalate-degrading taxa have been cultured and their oxalate-degrading capabilities quantified (2).
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