Abstract
Background SHC SH2 domain-binding protein 1 (SHCBP1), one of the members of Src homolog and collagen homolog (Shc) family, has been reported to be overexpressed in several malignant cancers and involved in tumor progression. However, the expression of SHCBP1 in nasopharyngeal carcinoma (NPC) remains unclear, and its clinical significance remains to be further elucidated. Methods The expression of SHCBP1 mRNA in 35 pair samples of NPC and adjacent normal tissues of NPC was detected by RT-qPCR. The expression level of SHCBP1 protein and mRNA in the selected cells was detected by western blot and RT-qPCR, respectively. The effects of SHCBP1 on NPC in vitro were observed by MTT method, colony formation assay, apoptosis assay, cell cycle assay, wound healing assay, transwell migration assay, and transwell invasion assay. Results SHCBP1 was highly expressed in clinical tissues and NPC cell lines, and SHCBP1 knockdown significantly inhibited NPC cell proliferation. Overexpression of SHCBP1 promoted NPC cell proliferation, migration, and invasion in NPC cell lines. Silencing SHCBP1 expression can delay cell cycle and inhibit cell apoptosis. Conclusion Our results suggest that SHCBP1 may promote proliferation and metastasis of NPC cells, which represents that SHCBP1 may act as a new indicator for predicting the prognosis of NPC and a new target for clinical treatment.
Highlights
Nasopharyngeal carcinoma (NPC) is one of the most common head and neck cancers in China
We found that SHCBP1 expression was higher in CNE-2Z and 5-8F cells compared with NP 69 cells (Figure 1(b)). e results showed that, in nasopharyngeal carcinoma (NPC) tissues and cell lines, the relative expression of SHCBP1 mRNA was significantly increased
We found that the expression of SHCBP1 was higher in CNE-2Z and 58F cells compared with that of NP 69 cells. ese results are consistent with previous studies that have shown that elevated SHCBP1 expression is detected in a variety of cancers and may have implications for early diagnosis [11,12,13,14,15]
Summary
Nasopharyngeal carcinoma (NPC) is one of the most common head and neck cancers in China. P66shc contains an amino terminal region (CH2), which plays an important role in the oxidative stress and apoptosis [6] Multiple signaling pathways such as insulin growth factor receptor (IGFR), insulin receptor (IR), fibroblast growth factor receptor (FGFR), and epidermal growth factor receptor (EGFR) can be activated by Shc [7]. SHC SH2 domain-binding protein 1 (SHCBP1), one of the members of Src homolog and collagen homolog (Shc) family, has been reported to be overexpressed in several malignant cancers and involved in tumor progression. SHCBP1 was highly expressed in clinical tissues and NPC cell lines, and SHCBP1 knockdown significantly inhibited NPC cell proliferation. Our results suggest that SHCBP1 may promote proliferation and metastasis of NPC cells, which represents that SHCBP1 may act as a new indicator for predicting the prognosis of NPC and a new target for clinical treatment
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