Abstract
Background Osteosarcoma (OS) is the most frequent bone tumor with high metastasis. This study is aimed at assessing the expression and prognostic significance of microRNA-1826 (miR-1826) in OS patients, as well as its biological function in tumor progression. Methods Quantitative Real-Time PCR was employed to measure the expression of miR-1826 in OS tissues and cell lines. Kaplan-Meier survival analysis and Cox regression model were used to evaluate the prognostic value of miR-1826. CCK-8 and Transwell assay were conducted to investigate the effect of miR-1826 on OS cell proliferation, migration, and invasion. Results miR-1826 expression was downregulated in OS tissues and cell lines and associated with OS patients' clinical stage and distant metastasis. Low levels of miR-1826 were related with shorter survival time and determined as an independent prognostic indicator for the overall survival of OS patients. The overexpression of miR-1826 in OS cells led to inhibited cell proliferation, migration, and invasion. Conclusion The decreased expression of miR-1826 predicts a poor prognosis in OS patients, and its overexpression inhibits OS cell proliferation, migration, and invasion. This newly identified miR-1826 provides a novel sight into the pathogenesis of OS and offers a candidate prognostic biomarker and therapeutic target for OS treatment.
Highlights
Osteosarcoma (OS) is the most frequent primary malignant bone tumor and predominantly occurs among children and adolescents aged 10-25 years, accounting for approximately 3.4% of all childhood tumors [1]
By investigating the expression of miR-1826 at the OS patients, we found that the expression of miR-1826 was significantly decreased in the tumor tissues compared with the adjacent normal tissues (P < 0:001, Figure 1(a))
The 122 OS patients were divided into IA-IIA stage (n = 57) and IIB-III stage (n = 65) according to the Enneking-Musculoskeletal Tumour Staging System, and the expression of miR-1826 was found to be downregulated in the IIB-III stage patients compared with the IAIIA stage patients (P < 0:05, Figure 1(b))
Summary
Osteosarcoma (OS) is the most frequent primary malignant bone tumor and predominantly occurs among children and adolescents aged 10-25 years, accounting for approximately 3.4% of all childhood tumors [1]. We International Journal of Genomics believed that the investigation of miRNAs for their clinical significance and biological function could improve the prognosis and treatment of OS. This study is aimed at assessing the expression and prognostic significance of microRNA-1826 (miR-1826) in OS patients, as well as its biological function in tumor progression. The decreased expression of miR-1826 predicts a poor prognosis in OS patients, and its overexpression inhibits OS cell proliferation, migration, and invasion. This newly identified miR-1826 provides a novel sight into the pathogenesis of OS and offers a candidate prognostic biomarker and therapeutic target for OS treatment
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