Down-Regulation of TSLP After EZH2 Silencing in ESCC Cell Line

Abstract

Background: Esophagus cancer is the sixth most common cause of death all over the world. This type of cancer is divided into two histological subtypes: Esophagus squamous cell carcinoma and Adeno-carcinoma, the first type of this type of cancer approximately include 90% of esophageal cancers in Asian countries. While radio therapy, chemotherapy and surgery are useful in treating, few people are survived. Therefore, it is need to molecular study to predict and prevent this cancer. EZH2 gene is a member of poly comb group proteins, and with other proteins of this group act as epigenetic regulators and have principle role in cellular functions. Objectives: In this study our aim was to evaluate the impact of EZH2 silencing on TSLP gene. Materials and Methods: For constantly silencing of EZH2 gene in KYSE-30, we used shRNA in retroviral vector. After viral production in HEK293T cell line, the cells of esophagus squamous cell carcinoma were transduced with viral packages. Silencing of EZH2 gene and the expression of TSLP were evaluated with real- time PCR. Results: By using retroviral EZH2 shRNA, the expression of EZH2 reduced over 2.5 fold change in KYSE-30. TSLP gene expression reduced over 2 fold change after EZH2 gene silencing in KYSE-30. Conclusions: Regarding the stablishing of KYSE-30 cell line with EZH2 silenced gene, Our results showed that the decrease of EZH2 gene expression cause change of downstream genes expression that will be eective on molecular treatment of ESCC.