Abstract
Patients with osteosarcoma (OS) usually have poor overall survival because of frequent metastasis. Long non-coding RNAs (lncRNAs) have been reported to be associated with tumorigenesis and metastasis. In this study, we investigated the expression and roles of lncRNA human histocompatibility leukocyte antigen (HLA) complex P5 (HCP5) in OS, aiming to provide a novel molecular mechanism for OS. HCP5 was up-regulated both in OS tissues and cell lines and high expression of HCP5 was associated to low survival in OS patients. Down-regulation of HCP5 inhibited cell proliferation, migration, and invasion, suggesting its carcinogenic role in OS. miR-101 was targeted by HCP5 and its expression was decreased in OS. The inhibitor of miR-101 reversed the impact of HCP5 down-regulation on cell proliferation, apoptosis, and metastasis in OS. Ephrin receptor 7 (EPHA7) was proved to be a target of miR-101 and had ability to recover the effects of miR-101 inhibitor in OS. In conclusion, lncRNA HCP5 knockdown suppressed cell proliferation, migration, and invasion, and induced apoptosis through depleting the expression of EPHA7 by binding to miR-101, providing a potential therapeutic strategy of HCP5 in OS.
Highlights
Osteosarcoma (OS) is an osteoid-producing highgrade malignancy of bone of mesenchymal origin [1]
HCP5 overexpression vector (HCP5) was up-regulated in OS tissues and cell lines First, we evaluated the expression of HCP5 in OS by Quantitative real-time polymerase chain reaction (qRT-PCR)
Multiple studies have reported that Long non-coding RNAs (lncRNAs) play specific roles in the development and metastasis of cancer, so characterization of cancerrelated lncRNAs and their target miRNAs involved in carcinogenesis attract more attention
Summary
Osteosarcoma (OS) is an osteoid-producing highgrade malignancy of bone of mesenchymal origin [1]. Human histocompatibility leukocyte antigen (HLA) complex P5 (HCP5) belongs to lncRNAs, and numerous reports have studied the functional roles of lncRNA HCP5 in cancers. HCP5 up-regulates runt-related transcription factor 1 (RUNX1) by acting as miR-139 sponge to promote astrocyte elevated gene-1 (AEG-1) expression, which is involved in several oncogenic effects in glioma cells [7]. Overexpressed HCP5 promotes the tumorigenesis of cervical cancer by enhancing metastasis associated in colon cancer (MACC1) expression by acting as miR-15a sponge [8]. These data reveal that HCP5 plays a circle role in the development of different cancers. Little study of HCP5 in OS has been reported
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