Abstract
Abstract A “Double-WURST” decoupling sequence is constructed for simultaneously decoupling the13CO and13Cαregions in15N/13C-double-labeled proteins. It uses frequency-shifted WURST decoupling combined with standard WURST decoupling. The power can be reduced by two-thirds compared to conventional WURST decoupling, which decouples the entire13C region including the unneeded region between13CO and13Cα. Well-decoupled spectra are acquired using the Double-WURST decoupling sequence with a test15N/13C-double-labeled peptide sample and 8 and 19.6 kDa15N/13C-double-labeled proteins. It has also been shown that adiabatic decoupling, WURST, for example, has already reached its highest decoupling indexn(=2) defined in a relation of decouple range Δfversus RF pulse strengthf1rms, i.e., Δf= λ(f1rms)n, where λ is a constant. Forngreater than 2, the energy conservation law would be violated, and therefore is not possible.
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