Abstract
• Immunochromatographic assay for the control of chicken muscles and eggs is proposed. • Chicken immunoglobulins of class Y (IgY) and ovalbumin (OVA) are biomarkers. • The test is based on the sandwich format with gold nanoparticles as antibody label. • LODs/cutoffs are 24.8/20 and 3.6/2 ng/mL for IgY and OVA; the assay time is 18 min. • The test system allows sensitive detecting analytes in raw meat mixtures and food. This study presents the development of a qualitative double immunochromatographic analysis (ICA) for the rapid simultaneous determination of ovalbumin (OVA) and immunoglobulins of class Y (IgY) of chicken origin. The latter serve as biomarkers to control the presence of chicken meat and eggs in food products. Polyclonal antibodies against OVA were produced in rabbits. The test system was based on the two-stage sandwich ICA with gold nanoparticles (AuNPs) as a label for anti-OVA and anti-IgY antibodies. Limits of detection (LODs)/cutoffs of IgY and OVA were 24.8/20 ng/mL and 3.6/2 ng/mL, respectively; the assay was performed within 18 min. The analysis allowed for the determination of OVA and IgY in raw meat mixtures containing chicken meat and dry egg white with visual cutoffs of 0.125 % and 0.0001 % (w/w) for chicken and egg white additives. The analysis in foodstuffs—ravioli with known composition—demonstrated that the developed double ICA can reveal IgY in poultry meat. The test system has a great potential for food product analysis when reliable determination and differentiation of chicken egg white and chicken meat as separate components are required.
Published Version
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