Abstract
The induction of renal metallothionein (MT) by indium and nickel loadings was studied by the use of a high performance liquid chromatograph coupled with an atomic absorption spectrophotometer. The amount of MT detected in the kidneys at 24h postinjection showed a linear dose-dependence up to 100 and 260μmol/kg body weight of indium and nickel, respectively, given by intraperitoneal (i.p.) injection. The results suggested that the renall MT was synthesized in the kidneys per se. The quantities of MT induced in the liver and kidneys by indium loading were correlated well with hepatic and renal indium concentrations, respectively, after i.p. or intravenous (i.v.) injection. Although the kidneys accumulated a high concentration of indium after repeated administrations, indium was not detected in the MT fraction on gel filtration chromatography. Only tiny MT peaks were observed at 7 d compared to those at 1 d after a single i.v. injection of indium.
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