Dose dependent interactions between estradiol‐17β and triiodothyronine with reference to different physiological and biochemical parameters on liver tissue of common Indian Toad (Bufo melanostictus)

Abstract

To study the dose dependent interaction between two chemically differentand functionally distinct hormones i.e. estradiol‐17β and tri‐iodothyronine(T3), six different animal groups of common Indian toad (Bufo melanostictus)were injected intramuscularly each with single dose of individual treatment[Estradiol‐17β (E‐40 and E‐80); T3 (T‐20 and T‐40), and combination dose (E‐40+T‐20 and E‐80+T‐40)]. The data clearly demonstrated the interaction between these two hormones with respect to different physiochemical parameters in the liver tissue, such as alkaline phosphates activity, oxygen consumption rate, lipid, cholesterol, RNA, DNA, protein, free amino acid, totalsugar and ascorbic acid content with respect to different days after treatment(0, 1, 5 and 10) as well as different doses of hormonal treatments, individuallyand in combination. The comparative study indicated the dose dependenthormone‐hormone interaction between estradiol‐17β and T3 hormone with respect to synergistic effect on oxygen consumption rate, fluctuation in lipid and cholesterol content, antagonistic effect of T3 on activation of the transcriptional apparatus to synthesize RNA, free amino acid metabolism, antagonistic effect on protein metabolism suggesting the protein catabolic or anabolic nature of T3, the permissive effect of estradiol‐17β upon T3 on sugar content in liver tissue of toad.