Abstract
Simple SummaryCordycepin is an adenosine analogue isolated from the fungus Cordyceps militaris. Cordycepin is a nucleoside antimetabolite that has shown a broad spectrum of biological activity including antineoplastic activity. limited research has been carried out on the effects of Cordycepin on the regenerative potential of stem cells, including dental pulp-derived mesenchymal stem cells. The present study was designed to assess if Cordycepin could enhance the vital properties of dental pulp-derived mesenchymal stem cells for regenerative purposes.Objective: To examine the effect of Cordycepin on the viability, proliferation, and migratory properties of dental pulp-derived mesenchymal stem cells. Materials and methods: The pulp was derived from human premolar teeth extracted for orthodontic purposes after obtaining informed consent. The samples were transferred to the laboratory for processing. DPSCs were expanded and characterized using flow cytometry and differentiation to the bone, adipose, and cartilage cells was examined. MTT Assay was performed using various concentrations of Cordycepin. The growth curve was plotted for 13 days. Cell cycle analysis was performed by flow cytometry. Migratory ability was assessed by wound healing assay. ROS generation was detected by flow cytometry. Gene expression was quantified by RT-qPCR. Statistical analysis was performed. p < 0.05 was considered as significant and p < 0.01 was considered as highly significant (* p < 0.05, and ** p < 0.01). Results: DPSCs expressed characteristic MSC-specific markers and trilineage differentiation. Cordycepin at lower concentrations did not affect the viability of DPSCs. The growth curve of cells showed a dose-dependent increase in cell numbers till the maximum dose. DPSCs treated with 2.5 µM Cordycepin was found to have a reduced G1 phase cell percentage. DPSCs treated with 2.5 µM and 5 µM Cordycepin showed a significant decrease in G2 phase cells. No significant difference was observed for S phase cells. Cordycepin treatment affected the migratory ability in DPSCs in a concentration-dependent manner. Conclusion: Cordycepin can be used at therapeutic doses to maintain stem cells.
Highlights
Biometabolites derived from plants, animals, fungi, and algae have been used in medicine throughout history
The present study examined the effects of Cordycepin on the viability, proliferation, and migratory characteristics of dental pulp stem cells
This study set out to establish the effects of various concentrations of Cordycepin on dental pulp stem cells (DPSC)
Summary
Biometabolites derived from plants, animals, fungi, and algae have been used in medicine throughout history. Plant and animal extracts have found use as preventive and therapeutic agents. The therapeutic effects of medicinal mushrooms have been recorded by various cultures, and have formed the basis for the synthesis of a large number of pharmaceutical products [1]. Cordycepin (Cpn), (3 -deoxyadenosine) is derived from Cordyceps militaris, a medicinal mushroom. Cordycepin has a broad spectrum of biological actions, including antineoplastic activity [2]. It resembles the nucleoside adenosine and is shown to kill neoplastic cells by interfering with their metabolism. Cordycepin has effects on the cardiovascular, respiratory, nervous, sexual, and immunological systems, and shows anti-oxidant, anti-inflammatory, and anti-microbial activities [3,4]
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