DOP023 Preconception care in IBD women leads to less disease relapses during pregnancy

Abstract

Background/Aims: An altered intestinal microbial composition (dysbiosis) is associated with intestinal inflammation in inflammatory bowel disease, which are characterized by decreased bacterial diversity and an altered ratio of beneficial and aggressive bacterial species. We analyzed intestinal microbial profiles of feces and mucosal tissues from Korean inflammatory bowel diseases (IBD) patients and healthy controls (HC) using high-throughput sequencing method. Methods: Fecal or mucosal tissue DNA were isolated from 35 Crohn's disease (CD) patients, 20 ulcerative colitis (UC) patients and 31 HC. Variable regions V1-V3 of the 16S rRNA gene were amplified from all samples. PCR products were sequenced using 454 GS FLX Titanium sequencing. The composition, diversity and richness of microbial communities were determined and compared among CD, UC and HC. Results: Intestinal microbial profile of fecal samples in Korean HC was similar toWestern HC data with predominant proportions of phyla Firmicutes and Bacteroidetes. Global structure and individual bacterial abundance were different between feces and ileal tissues in HC. Ileal tissues of active CD patients had higher level of phylum Proteobacteria, lower level of phylum Firmicutes, and decreased bacterial diversity compared to UC and HC. The proportion of phylum Fusobacteria was significantly higher in active CD patients compared to HC and CD patients in remission. No significant differences of mucosal bacterial composition between UC patients and HC. Conclusions: Our 16S rRNA sequence data demonstrates a community-level dysbiosis in Korean IBD patients. Fecal microbiota analysis does not effectively reflect ileal bacterial community structure. Intestinal microbial composition of UC tend to be closer to that of HC, suggesting different roles of intestinal microbiota in the pathogenesis of CD and UC. Acknowledgement: This study was supported by a grant of the Korean Health Technology R&D Project, Ministry of Health & Welfare, Republic of Korea. (A120176)