Abstract
The way DNA is packaged into chromatin influences gene function by regulating transcription, DNA replication, and recombination. Modifications of histones and chromatin remodeling mechanisms are critical for an organism's proper development. This research focuses on histone acetylation, a form of chromatin remodeling accomplished when protein complexes add acetyl groups to histone proteins, specifically the lysine residues on the N-terminal histone “tails”. The histone acetyltransferase GCN5 works with the transcriptional coactivator ADA2. Previous studies have shown that mutations in ADA2 or GCN5 effect normal growth, development, and gene expression patterns. In our work, we use Arabidopsis thaliana as an experimental model allowing us to study of the effects of chromatin modifying proteins on development. In Arabidopsis there are two genes, ADA2a and ADA2b, which resemble the Ada2 coactivator in yeast. Disruptions in ADA2b result in dwarfed plants but disruptions in ADA2a have no phenotypic effect. In this experiment recombinant DNA technology is used to create chimeric transgenic constructs in order to determine the domains of ADA2b necessary for normal phenotype and plant development.
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