Abstract
Objective: It has not been determined whether phototherapy (PT) has any effect on the induction of bilirubin conjugation. Serum conjugated bilirubin fractions were measured as an index of hepatic bilirubin conjugation to determine any effect of PT on bilirubin conjugation. Methods: Serum was sampled from 21 jaundiced (serum diazo bilirubin ≥274 μmol/L) term neonates prior to PT, and after 24 hours of PT. Unconjugated bilirubin(UCB), and mono- (MCB) and diconjugated (DCB) fractions of total conjugated bilirubin (TCB) were determined by alkaline methanolysis and reverse phase HPLC (results may be lower than diazo methods). Serum total bilirubin (STB), and MCB and DCB as%TCB, were calculated. Values [median (25-75% range)] we compared pre-PT to those at 24 hours by one way repeated measures analysis of variance on ranks. Significance was defined as p<0.05. Results:Table Conclusions: STB, UCB, and TCB decreased in parallel, maintaining a constant relationship of MCB and DCB to TCB. Preservation of constant relationships of the bilirubin fractions implies that PT does not alter bilirubin conjugation.
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