Does matrilin-3 gene polymorphism and primary knee osteoarthritis have a association in Indian population?

Abstract

INTRODUCTION: Osteoarthritis (OA) is estimated to be the fourth-leading cause of disability. Results from several studies have shown that OA is inherited and may vary by joint site. Twin family and studies have estimated the heritable component of OA to be between 50% and 65% with larger genetic influences for hand and hip OA than for knee OA. In view of the possible role of matrilin-3 (MATN-3) gene in primary OA, a number of studies during the past few years have evaluated the role of MATN-3 polymorphism in OA in various countries. SUBJECTS AND METHODS: The study was conducted in the Department of Orthopedics and Department of Bio-Technology in ERA's Lucknow Medical College and Hospital, Lucknow. Necessary blood (complete blood count, erythrocyte sedimentation rate, C reactive protein, rheumatoid arthritis factor, serum uric acid) and X-ray of the bilateral knee in investigations anteroposterior and lateral view on standing for confirmation of the diagnosis. Cases will be diagnosed with OA based on the Kellgren-LawrenceKL) Grading System. Five millimeters of venous blood will be drawn and poured in ethylenediaminetetraacetic acid-containing tubes. The DNA samples will be isolated from peripheral blood lymphocytes by the standard phenol extraction method. MATN-3 genotyping done from the blood. RESULTS: Statistically, the difference between the two groups was not significant (P = 0.603). No significant difference in genotype was observed among different KL-grades (P = 0.945). CONCLUSION: The findings of the present study showed that MATN-3 polymorphism does not seem to be associated with primary knee OA in the Indian population.