Does leaf mass per area (LMA) discriminate natural pine populations of different origins?

Abstract

Tree provenance trials are believed to be a valuable tool for assessing the adaptive potential of a population to a changing environment and ultimately for predicting the populations that are best adapted to global warming. Here, the phenotypic plasticity of morphometric traits of needles and lateral shoots of pines growing in a provenance plot in central Poland was examined to assess the inter- and intra-population variability. No significant differences were found in the measured and counted morphometric features, i.e., needle length (NL), cumulative needles length (CNL), thickness (ST), volume (SV) and shoot density (SD), number of needles per 5 cm fragment of shoot (NN), dry weight of needles (NDW) and shoot (SDW), thickness of bark (BT) and wood (WT), pith diameter (PD), and needle dry mass per area (LMA) among three pine populations while accounting for their region of origin (inter-population variability). In terms of the above-mentioned features, individual populations differed significantly from each other, except for NN and ST. We also noticed a positive, significant relationship between LMA and ST in all studied populations and based on Euclidean distances of measurable or counted traits, three population groups were identified. We concluded that LMA, which is commonly used to quantify leaf structure, is helpful in differentiating intra-population variability.