Abstract

Abstract The identification of plastid DNA markers that provide sufficient phylogenetic resolution at the species and population levels is an important challenge for plant systematics. This is mainly due to the relatively slow rate of evolution of the plastid genome. In this study, we examine the utility of several plastid DNA regions for phylogenetic inference at low taxonomic levels in tribe Lycieae (Solanaceae). The regions employed here previously provided considerable numbers of potentially informative characters in investigations of sequence variation across the plastid genome, and one region (trnH-psbA) has been advocated for use in DNA barcoding. We sequenced nine noncoding plastid DNA regions (∼8,400 bp) for a diverse sample of Lycium species and Phrodus microphyllus (tribe Lycieae), as well as the outgroup Nolana, and included published sequences from the distant outgroup Atropa. The nine regions varied with regard to their phylogenetic utility, as measured by the numbers of variable and parsimo...

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