Abstract
Objective: This study aimed to compare the level of reliability of three commercial real-time PCR kits in determining clinical samples.
 Methods: A total of 40 swabs samples which were previously tested positive, were re-test using the BioCov-19 RT-PCR kit, Sansure coronavirus disease 19 (COVID-19) nucleic acid diagnostic kit, and Kogen PowerCheck with Thermocycler (Roche). The amplification procedure is carried out based on the manual for each kit.
 Results: Sansure COVID-19 nucleic acid diagnostic was able to detect 40 samples with positive severe acute respiratory syndrome coronavirus 2 (SARS-COV-2) results detected in both genes, while the PowerChek™ 2019-nCoV real-time PCR kit able to detect 35 samples showed that SARS-COV-II was detected in both genes, and the BioCoV-19 RT-PCR Kit brand kit able to detect 34 samples showed positive SARS-COV-2 results in both genes.
 Conclusion: The three commercial kits show great ability detection, so that they can be used to detect the presence of SARS-COV-II in clinical samples, and also in mass screening.
Highlights
Since its first documented case, coronavirus disease 19 (COVID-19) has a massive spread throughout the continent and set its mark on the fourth populous country, Indonesia
Based on the SARS-COV-II detection results using three RT-Polymerase Chain reaction (PCR) kits, it was found that with the use of the PowerChekTM 2019-nCoV real-time PCR kit, 35 samples showed that SARS-CoV-2 was detected in both genes
Using Sansure COVID-19 nucleic acid diagnostic, there were 40 samples with positive SARSCoV-2 results detected in both genes (Table 2)
Summary
A total of 40 swabs samples which were previously tested positive, were re-test using the BioCov-19 RT-PCR kit, Sansure coronavirus disease 19 (COVID-19) nucleic acid diagnostic kit, and Kogen PowerCheck with Thermocycler (Roche). The amplification procedure is carried out based on the manual for each kit
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