Abstract
SUMMARY The DNA sequence homology relationships among six lepidopteran nuclear polyhedrosis viruses (NPVs) have been explored by hybridization of 32P-labelled NPV DNAs to Southern blots of restriction endonuclease-digested NPV DNA. The Autographa californica NPV (AcMNPV) shows extensive DNA sequence homology throughout the entire genome with the Rachiplusia ou NPV (RoMNPV). Both the Orgyia pseudotsugata NPV (OpMNPV) and the Porthetria dispar NPV (PdMNPV) share homologous regions, equivalent to 1% of the DNA genome, with AcMNPV and RoMNPV. This homology is localized in two regions on the AcMNPV physical map although other regions are also weakly homologous. Approx. 1% of the DNA of OpMNPV and PdMNPV show sequence homology with each other; the homology is primarily localized in two to four regions of the genomes. Heliothis zea NPV (HzSNPV) and Trichoplusia ni NPV (TnSNPV) share less than 0.2% sequence homology with the MNPVs and share less than 0.2% sequence homology with each other.
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