Abstract
SUMMARY To investigate the DNA sequence homology between the multicapsid nuclear polyhedrosis virus of Autographa californica and the multicapsid and single capsid nuclear polyhedrosis virus of Orgyia pseudotsugata, the stringency of hybridization conditions was varied. Thirteen to 25 % homology between the multicapsid viruses of A. californica and O. pseudotsugata was detected in 20, 30 and 40% formamide, indicating that fairly stable duplexes were being formed. Under the most stringent conditions in 50% formamide little homology was detected. In contrast to the multicapsid viruses, the single capsid virus of O. pseudotsugata demonstrated about 10% sequence homology in 20% formamide, but these duplexes were unstable and the percentage homology decreased markedly in the higher formamide concentrations. Examination of the specific regions of homology by hybridizing labelled DNA to Southern blots of the virus DNAs revealed that the regions of homology between these viruses were not limited to one region of the genome but were found on a number of restriction fragments.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
