Abstract
We introduce a nanoscale experimental platform that enables kinetic and equilibrium measurements of a wide range of molecular interactions by expanding the functionality of gel electrophoresis. Programmable, self-assembled DNA nanoswitches serve both as templates for positioning molecules, and as sensitive, quantitative reporters of molecular association and dissociation. We demonstrate this low cost, versatile, “lab-on-a-molecule” system by characterizing 10 different interactions, including a complex 4-body interaction with 5 discernable states.
Highlights
1657-Pos Board B608 Nanopore-Enhanced Positioning of Molecules in Zero-Mode Waveguides Joseph W
Currently there is no report about DNA dynamics near a nanopore
We introduce a nanoscale experimental platform that enables kinetic and equilibrium measurements of a wide range of molecular interactions by expanding the functionality of gel electrophoresis
Summary
1657-Pos Board B608 Nanopore-Enhanced Positioning of Molecules in Zero-Mode Waveguides Joseph W. 1655-Pos Board B606 Measurement of Salt Dependence of Single DNA Translocation through Si Nanopores with Ultraviolet Excitation Ito Shintaro, Hirohito Yamazaki, Mutsumi Tsukahara, Keiko Esashika, Toshiharu Saiki. Nanopore-based technologies have shown a great promise for developing single-molecule DNA sequencing. Optical detection requires to control DNA dynamics near solid-state nanopore.
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