DNA microarray analysis of dental pulp fibroblasts exfoliated from deciduous teeth

Abstract

Dental pulp plays an important role in tooth vitality. Previous studies have indicated that stem cells can be isolated from dental pulp, and dental pulp exfoliated from deciduous teeth has become a useful alternative for dental tissue engineering because of its higher proliferation rate. In the present study, we analyzed the differences in gene expressions between human dental pulps exfoliated from deciduous and permanent teeth by DNA microarray assays. A scatter plot of mRNA levels based on fluorescent signals in human dental pulps from deciduous and permanent teeth indicated a dispersed distribution pattern. In a scatter plot of the genes, 2,573 genes were expressed at >2-fold higher levels in dental pulp from deciduous teeth, compared with permanent teeth. To confirm the microarray results, insulin-like growth factorbinding protein 5 (IGFBP5) and vascular endothelial growth factor A (VEGFA) showing higher mRNA expression levels were selected and analyzed for their mRNA levels by RT-PCR. Both the IGFBP5 and VEGFA mRNA levels were upregulated by about 3-fold in dental pulp from deciduous teeth compared with permanent teeth. Thus, the RT-PCR results for these genes were consistent with the microarray data. The dental pulps in deciduous and permanent teeth differ significantly with regard to their developmental processes, tissue structures and functions. The present findings using DNA microarray analyses to detect differences in the gene expressions of deciduous and permanent teeth may be useful for dental pulp tissue engineering.