Abstract
BackgroundOur previous study found that chicken KLF7 was an important regulator in formation of adipose tissue. In the present study, we analyzed the association for DNA methylation in chicken KLF7 with its transcripts of abdominal adipose tissue and blood metabolic indicators.ResultsThe KLF7 transcripts of the adipose tissue of Chinese yellow broilers were associated with age (F = 6.67, P = 0.0035). In addition, the KLF7 transcripts were negatively correlated with blood glucose levels (r = − 0.61841, P = 0.0140). The DNA methylation levels of 26 CpG loci in the chicken KLF7 promoter and Exon 2 were studied by Sequenom MassArray. A total of 22 valid datasets were obtained. None of them was significantly different in relation to age (P > 0.05). However, the DNA methylation levels in the promoter were lower than those in Exon 2 (T = 40.74, P < 0.01). Correlation analysis showed that the DNA methylation levels of PCpG6 and E2CpG9 were significantly correlated with KLF7 transcripts and blood high-density lipoprotein levels, respectively, and many CpG loci were correlated with each other (P < 0.05). The methylation data were subjected to principal component analysis and factor analysis. The six principal components (z1–z6) were extracted and named Factors 1–6, respectively. Factor analysis showed that Factor 1 had a higher load on the loci in the promoter, and Factors 2–6 loaded highly on quite different loci in Exon 2. Correlation analysis showed that only z1 was significantly correlated to KLF7 transcripts (P < 0.05). In addition, an established regression equation between z1 and KLF7 transcripts was built, and the contribution of z1 to the variation on KLF7 transcripts was 34.29%.ConclusionsIn conclusion, the KLF7 transcripts of chicken abdominal adipose tissue might be inhibited by DNA methylation in the promoter, and it might be related to the DNA methylation level of PCpG6.
Highlights
Our previous study found that chicken Krüppel-like factor 7 (KLF7) was an important regulator in formation of adipose tissue
KLF7 mRNA expression of chicken adipose tissues The expression of KLF7 mRNA in the abdominal adipose tissue of Chinese fast-growing yellow broilers at the age of 2, 4, 6, and 8 weeks was studied by real-time PCR
The results showed that KLF7 mRNA was expressed in all the tissues studied
Summary
Our previous study found that chicken KLF7 was an important regulator in formation of adipose tissue. We analyzed the association for DNA methylation in chicken KLF7 with its transcripts of abdominal adipose tissue and blood metabolic indicators. The KLF7 gene is highly conserved among animals, especially mammalian species and birds. Reports on mammalian specie showed that KLF7 played an important role in the differentiation of neuroectodermal and mesodermal cell lineages in vitro [2]. KLF7 had been reported as a key regulator of human obesity [6], diabetes mellitus type 2 (T2DM) [7, 8], and blood disease [9]. Integrative genomic studies suggested that KLF7 might be one of the core factors that regulate cardiovascular diseases [16]
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