DNA MARKER-ASSISTED AND MORPHOLOGICAL SELECTION ON BC3 GENOTYPES SHORTCUT THE INTROGRESSION OF CMV TOLERANCE GENES ON CHILI PEPPER

Abstract

Normal 0 false false false EN-US X-NONE X-NONE Superior hybrid and CMV tolerant cultivar development requires a pair of high heterobeltiosis parent s and both of which are CMV tolerant. Gene introgression ha s to be accomplished if the tolerance does not exist in the parents. This research was objected to employ DNA markers and morphological traits on identifying the most resemble BC 3 CMV tolerant individuals to their recurrent parents to short cut the backcrossing cycle . This research us ed fifteen BC 3 populations generated from crosses of hybrid parents PBC1354 and PBC378 with toleran t parents of C1024, C1042 and C1043. The BC 3 populations were previously selected for their CMV tolerance and were characterized by RAPD technique and morphological traits . Selection by means of both RAPD marker and morphological traits identified BC3 individuals 99.9% resemble to their recurrent parent. Genotypes B3A24-20, B3A29-13, B3A29-22, B3B12-13, B3B37-9, B3B12-25, B3C16-5, B3C16-16, B3C34-18 99.9% were similar to PBC378, and B3D11-8, B3D11-17, B3D38-5, B3E12-17, B3E20-22, B3E31-19 were 99.9% similar to PBC1354. Those genotypes were both similar to their recurrent parent and tolerance to CMV. The employed strategy shortcut CMV tolerance genes introgression through a backcross breeding.